Nanowires create elite warriors to enhance T cell therapy

Adoptive T-cell therapy has revolutionized medicine. A patient's T-cells—a type of white blood cell that is part of the body's immune system—are extracted and modified in a lab and then infused back into the body, to seek and destroy infection, or cancer cells.

Now Georgia Tech bioengineer Ankur Singh and his research team have developed a method to improve this pioneering immunotherapy.

Their solution involves using nanowires to deliver therapeutic miRNA to T-cells. This new modification process retains the cells' naïve state, which means they'll be even better disease fighters when they're infused back into a patient.

"By delivering miRNA in naïve T cells, we have basically prepared an infantry, ready to deploy," Singh said. "And when these naïve cells are stimulated and activated in the presence of disease, it's like they've been converted into samurais."

Lean and mean

Currently in adoptive T-cell therapy, the cells become stimulated and preactivated in the lab when they are modified, losing their naïve state. Singh's new technique overcomes this limitation. The approach is described in a new study published in the journal Nature Nanotechnology.

"Naïve T-cells are more useful for immunotherapy because they have not yet been preactivated, which means they can be more easily manipulated to adopt desired therapeutic functions," said Singh, the Carl Ring Family Professor in the Woodruff School of Mechanical Engineering and the Wallace H. Coulter Department of Biomedical Engineering.

The raw recruits of the immune system, naïve T-cells are white blood cells that haven't been tested in battle yet. But these cellular recruits are robust, impressionable, and adaptable—ready and eager for programming.

"This process creates a well-programmed naïve T-cell ideal for enhancing immune responses against specific targets, such as tumors or pathogens," said Singh.

The precise programming naïve T-cells receive sets the foundational stage for a more successful disease fighting future, as compared to preactivated cells.

Giving fighter cells a boost

Within the body, naïve T-cells become activated when they receive a danger signal from antigens, which are part of disease-causing pathogens, but they send a signal to T-cells that activate the immune system.

Adoptive T-cell therapy is used against aggressive diseases that overwhelm the body's defense system. Scientists give the patient's T-cells a therapeutic boost in the lab, loading them up with additional medicine and chemically preactivating them.

That's when the cells lose their naïve state. When infused back into the patient, these modified T-cells are an effective infantry against disease—but they are prone to becoming exhausted. They aren't samurai. Naïve T-cells, though, being the young, programmable recruits that they are, could be.

The question for Singh and his team was: How do we give cells that therapeutic boost without preactivating them, thereby losing that pristine, highly suggestable naïve state? Their answer: Nanowires.

NanoPrecision: The pointed solution

Singh wanted to enhance naïve T-cells with a dose of miRNA. miRNA is a molecule that when used as a therapeutic, works as a kind of volume knob for genes, turning their activity up or down to keep infection and cancer in check. The miRNA for this study was developed in part by the study's co-author, Andrew Grimson of Cornell University.

"If we could find a way to forcibly enter the cells without damaging them, we could achieve our goal to deliver the miRNA into naïve T cells without preactivating them," Singh explained.